Computer-assisted instruction (CAI) is an alternative method for delivering educational material. CAI typically consists of videos or learning modules that require fairly inexpensive audio and visual capturing software to develop. CAI provides students with accessible, self-paced, affordable, and individualized instruction, while resolving limitations of physical lecture space and faculty availability. This presentation reveals the results of a comparative analysis of knowledge acquisition and retention in a physical agents course based upon method of delivery conducted by four graduate students in the DPT program. This research has been previously shared at Daemen College's 2014 DPT Research Symposium and the APTA's 2015 Combined Sections Meeting.